Transcriptional Potential Determines the Adaptability of Escherichia coli Strains with Different Fitness Backgrounds.

ABSTRACT: Adaptation through the fitness landscape may be influenced by the gene pool or expression network. However, genetic factors that determine the contribution of beneficial mutations during adaptive evolution are poorly understood. In this study, we experimentally evolved wild-type Escherichia coli K-12 MG1655 and its isogenic derivative that has two additional replication origins and shows higher background fitness. During the short time of experimental evolution, the fitness gains of the two E. coli strains with different fitness backgrounds converged. Populational genome sequencing revealed various mutations with different allele frequencies in evolved populations. Several mutations occurred in genes affecting transcriptional regulation (e.g., RNA polymerase subunit, RNase, ppGpp synthetas

SUBMITTER: Kim K 

PROVIDER: S-EPMC9769844 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature