Editing of a Specific Strain of Escherichia coli in the Mouse Gut Using Native Phages.

ABSTRACT: There is a lack of methodological investigation of the in situ functions of bacterial species in microecosystems. Here, we used native phages as a microbial editing tool for eliminating Escherichia coli strain MG1655 labeled with green fluorescent protein (GFP) in the mouse gut. The virulent phages (W1 and W3) possessed host specificity at both the genus and species levels, resulting in an 8.8-log₁₀ difference in the titer of viable bacteria after 12 h of phage treatment compared with that in the phage-free control in an in vitro test. In vivo, they reduced strain MG1655 colonizing the mouse gut at concentrations of 10⁶ to 10⁸ CFU g^-1 to a 10² CFU g^-1 level, which is almost undetectable by the plate colo

SUBMITTER: Ping L 

PROVIDER: S-EPMC9770003 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature