Lipopolysaccharide induced intestinal epithelial injury: a novel organoids-based model for sepsis in vitro.

ABSTRACT:

Background

Advances in organoid culture technology have provided a greater understanding of disease pathogenesis, which has been rarely studied in sepsis before. We aim to establish a suitable organoids-based intestinal injury model for sepsis.

Methods

Stable passaged organoids were constructed and pre-treated with lipopolysaccharide (LPS) to mimic sepsis-induced intestinal injury. The LPS-induced sepsis model was used as a reference. We used quantitative real-time polymerase chain reaction to evaluate the RNA levels of inflammatory factors and antimicrobial peptides. Enzyme-linked immunosorbent assay was used to evaluate the protein levels, hematoxylin and eosin staining was used to evaluate the pathology of the small intestine of mice, and immunohistochemistry and immunofl

SUBMITTER: Huang S 

PROVIDER: S-EPMC9771316 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature