Super-resolution microscopy reveals stochastic initiation of replication in Drosophila polytene chromosomes.

ABSTRACT: Studying the probability distribution of replication initiation along a chromosome is a huge challenge. Drosophila polytene chromosomes in combination with super-resolution microscopy provide a unique opportunity for analyzing the probabilistic nature of replication initiation at the ultrastructural level. Here, we developed a method for synchronizing S-phase induction among salivary gland cells. An analysis of the replication label distribution in the first minutes of S phase and in the following hours after the induction revealed the dynamics of replication initiation. Spatial super-resolution structured illumination microscopy allowed identifying multiple discrete replication signals and to investigate the behavior of replication signals in the first minutes of the S phase at the ultras

SUBMITTER: Kolesnikova TD 

PROVIDER: S-EPMC9771856 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature