Th17 Cell-Derived Amphiregulin Promotes Colitis-Associated Intestinal Fibrosis Through Activation of mTOR and MEK in Intestinal Myofibroblasts.

ABSTRACT:

Background & aims

Intestinal fibrosis is a significant complication of Crohn's disease (CD). Gut microbiota reactive Th17 cells are crucial in the pathogenesis of CD; however, how Th17 cells induce intestinal fibrosis is still not completely understood.

Methods

In this study, T-cell transfer model with wild-type (WT) and Areg^-/- Th17 cells and dextran sulfate sodium (DSS)-induced chronic colitis model in WT and Areg^-/- mice were used. CD4⁺ T-cell expression of AREG was determined by quantitative reverse-transcriptase polymerase chain reaction and enzyme-linked immunosorbent assay. The effect of AREG on proliferation/migration/collagen expression in human intestinal myofibroblasts was determined. AREG expression was assessed in healthy control

SUBMITTER: Zhao X 

PROVIDER: S-EPMC9772145 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature