Hepatocyte Adenosine Kinase Promotes Excessive Fat Deposition and Liver Inflammation.

ABSTRACT:

Background & aims

Nonalcoholic fatty liver disease is highly associated with obesity and progresses to nonalcoholic steatohepatitis when the liver develops overt inflammatory damage. While removing adenosine in the purine salvage pathway, adenosine kinase (ADK) regulates methylation reactions. We aimed to study whether hepatocyte ADK functions as an obesogenic gene/enzyme to promote excessive fat deposition and liver inflammation.

Methods

Liver sections of human subjects were examined for ADK expression using immunohistochemistry. Mice with hepatocyte-specific ADK disruption or overexpression were examined for hepatic fat deposition and inflammation. Liver lipidomics, hepatocyte RNA sequencing (RNA-seq), and single-cell RNA-seq for liver nonparenchymal cells were performed t

SUBMITTER: Li H 

PROVIDER: S-EPMC9772177 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature