An established protocol for generating transgenic wheat for wheat functional genomics via particle bombardment.

ABSTRACT: Wheat is one of the most important food crops in the world and is considered one of the top targets in crop biotechnology. With the high-quality reference genomes of wheat and its relative species and the recent burst of genomic resources in Triticeae, demands to perform gene functional studies in wheat and genetic improvement have been rapidly increasing, requiring that production of transgenic wheat should become a routine technique. While established for more than 20 years, the particle bombardment-mediated wheat transformation has not become routine yet, with only a handful of labs being proficient in this technique. This could be due to, at least partly, the low transformation efficiency and the technical difficulties. Here, we describe the current version of this method through adapt

SUBMITTER: Wang Y 

PROVIDER: S-EPMC9772560 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature