Urea is a drop-in nitrogen source alternative to ammonium sulphate in Yarrowia lipolytica.

ABSTRACT: Media components, including the nitrogen source, are significant cost factors in cultivation processes. The nitrogen source also influences cell behavior and production performance. Ammonium sulfate is a widely used nitrogen source for microorganisms' cultivation. Urea is a sustainable and cheap alternative nitrogen source. We investigated the influence of urea as a nitrogen source compared to ammonium sulfate by cultivating phenotypically different Yarrowia lipolytica strains in chemostats under carbon or nitrogen limitation. We found no significant coherent changes in growth and lipid production. RNA sequencing revealed no significant concerted changes in the transcriptome. The genes involved in urea uptake and degradation are not upregulated on a transcriptional level. Our findin

SUBMITTER: Konzock O 

PROVIDER: S-EPMC9772842 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature