Inhibition of SND1 overcomes chemoresistance in bladder cancer cells by promoting ferroptosis.

ABSTRACT: Chemotherapy remains one of the most important adjuvant treatments for bladder cancer (BC). However, similar to other malignancies, BC is prone to chemotherapy resistance and only approximately half of muscle‑invasive patients with BC respond to chemotherapy. The present study aimed to reveal the mechanisms underlying chemoresistance in BC cells. Cell viabilities were assessed by CCK‑8 assay. The differentiated expression of genes in chemoresistant and their parental BC cells were examined by RNA sequencing. Cell death was determined by flow cytometry. Different cell death inhibitors were used to determine the types of cell death. Levels of reactive oxygen species, iron, glutathione and malondialdehyde were assessed using the corresponding commercial kits. ChIP and dual luciferase activity

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC9773013 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature