Bacterial ribosomal RNA detection in cerebrospinal fluid using a viromics approach.

ABSTRACT:

Background

In patients with central nervous system (CNS) infections identification of the causative pathogen is important for treatment. Metagenomic next-generation sequencing techniques are increasingly being applied to identify causes of CNS infections, as they can detect any pathogen nucleic acid sequences present. Viromic techniques that enrich samples for virus particles prior to sequencing may simultaneously enrich ribosomes from bacterial pathogens, which are similar in size to small viruses.

Methods

We studied the performance of a viromic library preparation technique (VIDISCA) combined with low-depth IonTorrent sequencing (median ~ 25,000 reads per sample) for detection of ribosomal RNA from common pathogens, analyzing 89 cerebrospinal fluid samples from patients wi

SUBMITTER: Kinsella CM 

PROVIDER: S-EPMC9773461 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature