Identification of biological pathways and processes regulated by NEK5 in breast epithelial cells via an integrated proteomic approach.

ABSTRACT: Specific members of the Nima-Related Kinase (NEK) family have been linked to cancer development and progression, and a role for NEK5, one of the least studied members, in breast cancer has recently been proposed. However, while NEK5 is known to regulate centrosome separation and mitotic spindle assembly, NEK5 signalling mechanisms and function in this malignancy require further characterization. To this end, we established a model system featuring overexpression of NEK5 in the immortalized breast epithelial cell line MCF-10A. MCF-10A cells overexpressing NEK5 exhibited an increase in clonogenicity under monolayer conditions and enhanced acinar size and abnormal morphology in 3D Matrigel culture. Interestingly, they also exhibited a marked reduction in Src activation and downstream signalli

SUBMITTER: de Castro Ferezin C 

PROVIDER: S-EPMC9773587 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature