Parallel single-cell and bulk transcriptome analyses reveal key features of the gastric tumor microenvironment.

ABSTRACT:

Background

The tumor microenvironment (TME) has been shown to strongly influence treatment outcome for cancer patients in various indications and to influence the overall survival. However, the cells forming the TME in gastric cancer have not been extensively characterized.

Results

We combine bulk and single-cell RNA sequencing from tumors and matched normal tissue of 24 treatment-naïve GC patients to better understand which cell types and transcriptional programs are associated with malignant transformation of the stomach. Clustering 96,623 cells of non-epithelial origin reveals 81 well-defined TME cell types. We find that activated fibroblasts and endothelial cells are most prominently overrepresented in tumors. Intercellular network reconstruction and survival analysis of

SUBMITTER: Kang B 

PROVIDER: S-EPMC9773611 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature