A cross-platform approach to characterize and screen potential neurovascular unit toxicants.

ABSTRACT: Development of the neurovascular unit (NVU) is a complex, multistage process that requires orchestrated cell signaling mechanisms across several cell types and ultimately results in formation of the blood-brain barrier. Typical high-throughput screening (HTS) assays investigate single biochemical or single cell responses following chemical insult. As the NVU comprises multiple cell types interacting at various stages of development, a methodology combining high-throughput results across pertinent cell-based assays is needed to investigate potential chemical-induced disruption to the development of this complex cell system. To this end, we implemented a novel method for screening putative NVU disruptors across diverse assay platforms to predict chemical perturbation of the developing NVU. H

SUBMITTER: Zurlinden TJ 

PROVIDER: S-EPMC9773816 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature