A Defucosylated Anti-EpCAM Monoclonal Antibody (EpMab-37-mG_2a-f) Exerts Antitumor Activity in Xenograft Model.

ABSTRACT: The epithelial cell adhesion molecule (EpCAM) is a stem cell and carcinoma antigen, which mediates cellular adhesion and proliferative signaling by the proteolytic cleavage. In contrast to low expression in normal epithelium, EpCAM is frequently overexpressed in various carcinomas, which correlates with poor prognosis. Therefore, EpCAM has been considered as a promising target for tumor diagnosis and therapy. Using the Cell-Based Immunization and Screening (CBIS) method, we previously established an anti-EpCAM monoclonal antibody (EpMab-37; mouse IgG₁, kappa). In this study, we investigated the antibody-dependent cellular cytotoxicity (ADCC), complement-dependent cytotoxicity (CDC), and an antitumor activity by a defucosylated mouse IgG_2a-type of EpMab-37 (EpMab-37-mG

SUBMITTER: Asano T 

PROVIDER: S-EPMC9774109 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature