New Insights into the Mechanism of Antibacterial Action of Synthetic Peptide Mo-CBP₃-PepI against Klebsiella pneumoniae.

ABSTRACT: Klebsiella pneumoniae is a multidrug-resistant opportunistic human pathogen related to various infections. As such, synthetic peptides have emerged as potential alternative molecules. Mo-CBP₃-PepI has presented great activity against K. pneumoniae by presenting an MIC₅₀ at a very low concentration (31.25 µg mL^-1). Here, fluorescence microscopy and proteomic analysis revealed the alteration in cell membrane permeability, ROS overproduction, and protein profile of K. pneumoniae cells treated with Mo-CBP₃-PepI. Mo-CBP₃-PepI led to ROS overaccumulation and membrane pore formation in K. pneumoniae cells. Furthermore, the proteomic analysis highlighted changes in essential metabolic pathways.

SUBMITTER: Branco LAC 

PROVIDER: S-EPMC9774128 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature