Improving Reproducibility to Enhance Scientific Rigor through Consideration of Mouse Diet.

ABSTRACT: Animal husbandry conditions, including rodent diet, constitute an example highlighting the importance of reporting experimental variables to enhance scientific rigor. In the present study, we examine the effects of three common rodent diets including two chows (Purina 5015 and Teklad 2019) and one purified ingredient diet (AIN-76A) on growth anthropometrics (body weight), behavior (nest building, actigraphy, passive avoidance) and blood biomarkers (ketones, glucose, amino acid profiles) in male and female C57BL/6J mice. We find increased body weight in response to the chows compared to purified ingredient diet albeit selectively in male mice. We did not find significantly altered behavior in female or male wild type C57BL/6J mice. However, amino acid profiles changed as an effect of sex an

SUBMITTER: Westmark CJ 

PROVIDER: S-EPMC9774320 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature