Discovery of α-l-Glucosidase Raises the Possibility of α-l-Glucosides in Nature.

ABSTRACT: Glucose, a common monosaccharide in nature, is dominated by the d-enantiomer. Meanwhile, the discovery of l-glucose-utilizing bacteria and the elucidation of their metabolic pathways 10 years ago suggests that l-glucose exists naturally. Most carbohydrates exist as glycosides rather than monosaccharides; therefore, we expected that nature also contains l-glucosides. Sequence analysis within glycoside hydrolase family 29 led us to identify two α-l-glucosidases, ClAgl29A and ClAgl29B, derived from Cecembia lonarensis LW9. ClAgl29A and ClAgl29B exhibited higher K _m, k _cat, and k _cat/K _m values for p-nitrophenyl α-l-glucoside than that for p-nitrophenyl α-l-fucoside. Structural analysis of ClAgl29B in

SUBMITTER: Shishiuchi R 

PROVIDER: S-EPMC9774334 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature