Modification of Fatty Acid Composition of Escherichia coli by Co-Expression of Fatty Acid Desaturase and Thioesterase from Arabidopsis thaliana.

ABSTRACT: Fatty acid composition has an important influence on the fluidity of biological membranes, which is a key factor for the survival of Escherichia coli. With the aim to modify fatty acid composition in this experimentally friendly microorganism, the AtFab2 gene, encoding the Arabidopsis thaliana fatty acid desaturase, was expressed separately and jointly with AtFatA, a fatty acid thioesterase of the same plant origin. The expression of ATFab2 desaturase resulted in an enhancement of cis-vaccenic acid (18:1Δ11) contents, while amounts of palmitioleic acid (16:1Δ9) accumulated by E. coli were increased by 130% for the expression of the AtFatA thioesterase. In the final engineered strain co-expressing AtFab2 and AtFatA, the percentage of palmitic acid (

SUBMITTER: Pu Y 

PROVIDER: S-EPMC9774610 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature