Influence of Centrifugation and Shaking on the Self-Assembly of Lysozyme Fibrils.

ABSTRACT: Protein self-assembly into fibrils and oligomers plays a key role in the etiology of degenerative diseases. Several pathways for this self-assembly process have been described and shown to result in different types and ratios of final assemblies, therewith defining the effective physiological response. Known factors that influence assembly pathways are chemical conditions and the presence or lack of agitation. However, in natural and industrial systems, proteins are exposed to a sequence of different and often complex mass transfers. In this paper, we compare the effect of two fundamentally different mass transfer processes on the fibrilization process. Aggregation-prone solutions of hen egg white lysozyme were subjected to predominantly non-advective mass transfer by employing centrifugat

SUBMITTER: Krzek M 

PROVIDER: S-EPMC9775142 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature