Quantitative Aspect of Bacillus subtilis σB Regulatory Network-A Computational Simulation.

ABSTRACT: Bacillus subtilis is a model organism used to study molecular processes in prokaryotic cells. Sigma factor B, which associates with RNA polymerase, is one of the transcriptional regulators involved in the cell's response to environmental stress. This study addresses the key question of how the levels of free SigB, which acts as the actual regulator of gene expression, are controlled. A set of chemical equations describing the network controlling the levels of free SigB was designed, leading to a set of differential equations quantifying the dynamics of the network. Utilizing a microarray-measured gene expression time series then allowed the simulation of the kinetic behavior of the network in real conditions and investigation of the role of phosphatases RsbU/RsbP transmitting the en

SUBMITTER: Vohradsky J 

PROVIDER: S-EPMC9775250 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature