Influence of Protein Carbonylation on Human Adipose Tissue Dysfunction in Obesity and Insulin Resistance.

ABSTRACT:

Background

Obesity is characterized by adipose tissue dysregulation and predisposes individuals to insulin resistance and type 2 diabetes. At the molecular level, adipocyte dysfunction has been linked to obesity-triggered oxidative stress and protein carbonylation, considering protein carbonylation as a link between oxidative stress and metabolic dysfunction. The identification of specific carbonylated proteins in adipose tissue could provide novel biomarkers of oxidative damage related to metabolic status (i.e prediabetes). Thus, we aimed at characterizing the subcutaneous and omental human adipose tissue carbonylome in obesity-associated insulin resistance.

Methods

2D-PAGE was used to identify carbonylated proteins, and clinical correlations studies and molecular biology a

SUBMITTER: Navarro-Ruiz MC 

PROVIDER: S-EPMC9775537 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature