Gabapentin Disrupts Binding of Perlecan to the α₂δ₁ Voltage Sensitive Calcium Channel Subunit and Impairs Skeletal Mechanosensation.

ABSTRACT: Our understanding of how osteocytes, the principal mechanosensors within bone, sense and perceive force remains unclear. Previous work identified "tethering elements" (TEs) spanning the pericellular space of osteocytes and transmitting mechanical information into biochemical signals. While we identified the heparan sulfate proteoglycan perlecan (PLN) as a component of these TEs, PLN must attach to the cell surface to induce biochemical responses. As voltage-sensitive calcium channels (VSCCs) are critical for bone mechanotransduction, we hypothesized that PLN binds the extracellular α₂δ₁ subunit of VSCCs to couple the bone matrix to the osteocyte membrane. Here, we showed co-localization of PLN and α₂δ₁ along osteocyte dendritic processes. Additio

SUBMITTER: Reyes Fernandez PC 

PROVIDER: S-EPMC9776037 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature