Transplantation of Skeletal Muscle-Derived Sca-1⁺/PW1⁺/Pax7^- Interstitial Cells (PICs) Improves Cardiac Function and Attenuates Remodeling in Mice Subjected to Myocardial Infarction.

ABSTRACT: We have previously shown that skeletal muscle-derived Sca-1⁺/PW1⁺/Pax7^- interstitial cells (PICs) are multi-potent and enhance endogenous repair and regeneration. Here, we investigated the regenerative potential of PICs following intramyocardial transplantation in mice subjected to an acute myocardial infarction (MI). MI was induced through the ligation of the left anterior descending coronary artery in 8-week old male C57BL/6 mice. 5 × 10⁵ eGFP-labelled PICs (MI + PICs; n = 7) or PBS (MI-PBS; n = 7) were injected intramyocardially into the border zone. Sham mice (n = 8) were not subjected to MI, or the transplantation of PICs or PBS. BrdU was administered via osmotic mini-pump for 14 days. Echocardiography was performed prior to surgery (baselin

SUBMITTER: Ruchaya PJ 

PROVIDER: S-EPMC9776789 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature