Aldosterone up-regulates basolateral Na⁺ -K⁺ -2Cl^- cotransporter-1 to support enhanced large-conductance K⁺ channel-mediated K⁺ secretion in rat distal colon.

ABSTRACT: Na⁺ -K⁺ -2Cl^- cotransporter-1 (NKCC1) facilitates basolateral K⁺ and Cl^- uptake, supporting their efflux across mucosal membranes of colonic epithelial cells. NKCC1 activity has also been shown to be critical for electrogenic K⁺ secretion induced by aldosterone, which is known to stimulate large-conductance K⁺ (BK) channel expression in mucosal membranes. This study was aimed to (1) identify whether aldosterone enhances NKCC1 expression specifically to support BK-mediated K⁺ secretion and (2) to determine whether increased NKCC1 supports electrogenic Cl^- secretion in parallel to K⁺ secretion. Dietary Na⁺ depletion was used to induce secondary hyperaldosteronism in rats,

SUBMITTER: Nickerson AJ 

PROVIDER: S-EPMC9777186 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature