Comparison of Metagenomics and Metatranscriptomics Tools: A Guide to Making the Right Choice.

ABSTRACT: The study of microorganisms is a field of great interest due to their environmental (e.g., soil contamination) and biomedical (e.g., parasitic diseases, autism) importance. The advent of revolutionary next-generation sequencing techniques, and their application to the hypervariable regions of the 16S, 18S or 23S ribosomal subunits, have allowed the research of a large variety of organisms more in-depth, including bacteria, archaea, eukaryotes and fungi. Additionally, together with the development of analysis software, the creation of specific databases (e.g., SILVA or RDP) has boosted the enormous growth of these studies. As the cost of sequencing per sample has continuously decreased, new protocols have also emerged, such as shotgun sequencing, which allows the profiling of all taxonomic

SUBMITTER: Terron-Camero LC 

PROVIDER: S-EPMC9777648 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature