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Solubilities of Amino Acids in Aqueous Solutions of Chloride or Nitrate Salts of Divalent (Mg²⁺ or Ca²⁺) Cations.

ABSTRACT: The solubilities of glycine, l-leucine, l-phenylalanine, and l-aspartic acid were measured in aqueous MgCl₂, Mg(NO₃)₂, CaCl_2,, and Ca(NO₃)₂ solutions with concentrations ranging from 0 to 2 mol/kg at 298.2 K. The isothermal analytical method was used combined with the refractive index measurements for composition analysis guaranteeing good accuracy. All salts induced a salting-in effect with a higher magnitude for those containing the Ca²⁺ cation. The nitrate anions also showed stronger binding with the amino acids, thus increasing their relative solubility more than the chloride anions. In particular, calcium nitrate induces an increase in the amino acid solubility from 2.4 (glycine) to 4.6 fold (l-aspartic acid) compared to the corresponding value in water. Amino acid solubility data in aqueous MgCl₂ and CaCl₂ solutions collected from the open literature were combined with that from this work, allowing us to analyze the relations between the amino acid structure and the salting-in magnitude.

SUBMITTER: Aliyeva M

PROVIDER: S-EPMC9777878 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature

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Solubilities of Amino Acids in Aqueous Solutions of Chloride or Nitrate Salts of Divalent (Mg2+ or Ca2+) Cations.

Aliyeva Mehriban M Brandão Paula P Gomes José R B JRB Coutinho João A P JAP Ferreira Olga O Pinho Simão P SP

Journal of chemical and engineering data 20220518 6

The solubilities of glycine, l-leucine, l-phenylalanine, and l-aspartic acid were measured in aqueous MgCl2, Mg(NO3)2, CaCl2,, and Ca(NO3)2 solutions with concentrations ranging from 0 to 2 mol/kg at 298.2 K. The isothermal analytical method was used combined with the refractive index measurements for composition analysis guaranteeing good accuracy. All salts induced a salting-in effect with a higher magnitude for those containing ...[more]

PMID: 36568723

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Project description:Divalent cations, especially Ca2+ and Mg2+, play a vital role in the function of biomolecules and making them important to be constituents in samples for in vitro biophysical and biochemical characterizations. Although lipid nanodiscs are becoming valuable tools for structural biology studies on membrane proteins and for drug delivery, most types of nanodiscs used in these studies are unstable in the presence of divalent metal ions. To avoid the interaction of divalent metal ions with the belt of the nanodiscs, synthetic polymers have been designed and demonstrated to form stable lipid nanodiscs under such unstable conditions. Such polymer-based nanodiscs have been shown to provide an ideal platform for structural studies using both solid-state and solution NMR spectroscopies because of the near-native cell-membrane environment they provide and the unique magnetic-alignment behavior of large-size nanodiscs. In this study, we report an investigation probing the effects of Ca2+ and Mg2+ ions on the formation of polymer-based lipid nanodiscs and the magnetic-alignment properties using a synthetic polymer, styrene maleimide quaternary ammonium (SMA-QA), and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) lipids. Phosphorus-31 NMR experiments were used to evaluate the stability of the magnetic-alignment behavior of the nanodiscs for varying concentrations of Ca2+ or Mg2+ at different temperatures. It is remarkable that the interaction of divalent cations with lipid headgroups promotes the stacking up of nanodiscs that results in the enhanced magnetic alignment of nanodiscs. Interestingly, the reported results show that both the temperature and the concentration of divalent metal ions can be optimized to achieve the optimal alignment of nanodiscs in the presence of an applied magnetic field. We expect the reported results to be useful in the design of nanodisc-based nanoparticles for various applications in addition to atomic-resolution structural and dynamics studies using NMR and other biophysical techniques.

Dataset Information

Solubilities of Amino Acids in Aqueous Solutions of Chloride or Nitrate Salts of Divalent (Mg²⁺ or Ca²⁺) Cations.

Publications

Solubilities of Amino Acids in Aqueous Solutions of Chloride or Nitrate Salts of Divalent (Mg<sup>2+</sup> or Ca<sup>2+</sup>) Cations.

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