Proteomic Profiling of Major Peanut Allergens and Their Post-Translational Modifications Affected by Roasting.

ABSTRACT: Post-translational modifications (PTMs) are covalent changes occurring on amino acid side chains of proteins and yet are neglected structural and functional aspects of protein architecture. The objective was to detect differences in PTM profiles that take place after roasting using open PTM search. We conducted a bottom-up proteomic study to investigate the impact of peanut roasting on readily soluble allergens and their PTM profiles. Proteomic PTM profiling of certain modifications was confirmed by Western blotting with a series of PTM-specific antibodies. In addition to inducing protein aggregation and denaturation, roasting may facilitate change in their PTM pattern and relative profiling. We have shown that Ara h 1 is the most modified major allergen in both samples in terms of modific

SUBMITTER: Đukic T 

PROVIDER: S-EPMC9778155 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature