Engineering the Thermostability of the Mono- and Diacylglycerol Lipase SMG1 for the Synthesis of Diacylglycerols.

ABSTRACT: Diacylglycerols (DAGs) display huge application prospectives in food industries. Therefore, new strategies to produce diacylglycerides are needed. Malassezia globose lipase (SMG1) could be used to synthesize DAGs. However, the poor thermostability of SMG1 seriously hampers its application. Herein, a rational design was used to generate a more thermostable SMG1. Compared with the wild type (WT), the M5D mutant (Q34P/A37P/M176V/G177A/M294R/ G28C-P206C), which contains five single-point mutations and one additional disulfide bond, displayed a 14.0 °C increase in the melting temperature (T_m), 5 °C in the optimal temperature, and 1154.3-fold in the half-life (t_1/2) at 55 °C. Meanwhile, the specific activity towards DAGs of the M5D variant was improved

SUBMITTER: Li L 

PROVIDER: S-EPMC9778158 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature