Effects of Salinity and Temperature on the Flexibility and Function of a Polyextremophilic Enzyme.

ABSTRACT: The polyextremophilic β-galactosidase enzyme of the haloarchaeon Halorubrum lacusprofundi functions in extremely cold and hypersaline conditions. To better understand the basis of polyextremophilic activity, the enzyme was studied using steady-state kinetics and molecular dynamics at temperatures ranging from 10 °C to 50 °C and salt concentrations from 1 M to 4 M KCl. Kinetic analysis showed that while catalytic efficiency (k_cat/K_m) improves with increasing temperature and salinity, K_m is reduced with decreasing temperatures and increasing salinity, consistent with improved substrate binding at low temperatures. In contrast, k_cat was similar from 2-4 M KCl across the temperature range, with the calculated enthal

SUBMITTER: Laye VJ 

PROVIDER: S-EPMC9779221 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature