Direct or Indirect ESPT Mechanism in CFP psamFP488? A Theoretical-Computational Investigation.

ABSTRACT: Fluorescent Proteins are widely studied for their multiple applications in technological and biotechnological fields. Despite this, they continue to represent a challenge in terms of a complete understanding of all the non-equilibrium photo-induced processes that rule their properties. In this context, a theoretical-computational approach can support experimental results in unveiling and understanding the processes taking place after electronic excitation. A non-standard cyan fluorescent protein, psamFP488, is characterized by an absorption maximum that is blue-shifted in comparison to other cyan fluorescent proteins. This protein is characterized by an extended Stokes shift and an ultrafast (170 fs) excited state proton transfer. In this work, a theoretical-computational study, including

SUBMITTER: Donati G 

PROVIDER: S-EPMC9779432 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature