Cellular features of localized microenvironments in human meniscal degeneration: a single-cell transcriptomic study.

ABSTRACT:

Background

Musculoskeletal tissue degeneration impairs the life quality and function of many people. Meniscus degeneration is a major origin of knee osteoarthritis and a common threat to athletic ability, but its cellular mechanism remains elusive.

Methods

We built a cell atlas of 12 healthy or degenerated human meniscus samples from the inner and outer meniscal zones of 8 patients using scRNA-seq to investigate meniscal microenvironment homeostasis and its changes in the degeneration process and verified findings with immunofluorescent imaging.

Results

We identified and localized cell types in inner and outer meniscus and found new chondrocyte subtypes associated with degeneration. The observations suggested understandings on how cellular compositions, functions, and

SUBMITTER: Fu W 

PROVIDER: S-EPMC9779791 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature