Cell-Laden Gradient Microgel Suspensions for Spatial Control of Differentiation During Biofabrication.

ABSTRACT: During tissue development, stem and progenitor cells form functional tissue with high cellular diversity and intricate micro- and macro-architecture. Current approaches have attempted to replicate this process with materials cues or through spontaneous cell self-organization. However, cell-directed and materials-directed organization are required simultaneously to achieve biomimetic structure and function. Here, it is shown how integrating live adipose derived stem cells with gradient microgel suspensions steers divergent differentiation outcomes. Microgel matrices composed of small particles are found to promote adipogenic differentiation, while larger particles fostered increased cell spreading and osteogenic differentiation. Tuning the matrix formulation demonstrates that early cell adh

SUBMITTER: Molley TG 

PROVIDER: S-EPMC9780160 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature