Endogenous SOD2 (Superoxide Dismutase) Regulates Platelet-Dependent Thrombin Generation and Thrombosis During Aging.

ABSTRACT:

Background

Reactive oxygen species (ROS) contribute to platelet hyperactivation during aging. Several oxidative pathways and antioxidant enzymes have been implicated; however, their mechanistic contributions during aging remain elusive. We hypothesized that mitochondria are an important source of platelet ROS and that mitochondrial SOD2 (superoxide dismutase) protects against mitochondrial ROS-driven platelet activation and thrombosis during aging.

Methods

We studied littermates of platelet-specific SOD2-knockout (SOD2^fl/flPf4Cre, pSOD2-KO) and control (SOD2^fl/fl) mice at young (4-5 months) or old (18-20 months) ages. We examined agonist-induced platelet activation, platelet-dependent thrombin generation potential, and susceptibility to in vivo thrombos

SUBMITTER: Sonkar VK 

PROVIDER: S-EPMC9780178 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature