Donor chimerism and immune reconstitution following haploidentical transplantation in sickle cell disease.

ABSTRACT:

Introduction

We previously reported the initial results of a phase II multicenter transplant trial using haploidentical parental donors for children and aolescents with high-risk sickle cell disease achieving excellent survival with exceptionally low rates of graft-versus-host disease and resolution of sickle cell disease symptoms. To investigate human leukocyte antigen (HLA) sensitization, graft characteristics, donor chimerism, and immune reconstitution in these recipients.

Methods

CD34 cells were enriched using the CliniMACS^® system with a target dose of 10 x 10⁶ CD34⁺ cells/kg with a peripheral blood mononuclear cell (PBMNC) addback dose of 2x10⁵ CD3/kg in the final product. Pre-transplant HLA antibodies were characterized. Don

SUBMITTER: Chu Y 

PROVIDER: S-EPMC9780682 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature