Stability Enhancement of Freeze-Dried Gelatin/Alginate Coacervates for bFGF Delivery.

ABSTRACT: Chronic wound sites have elevated levels of proteolytic enzymes that negate the activity of topically applied growth factors. bFGF encapsulated in gelatin/alginate coacervates was protected from protease and showed better activity than bFGF in solution; however, its activity decreased with particle size and PDI increase after freeze-drying and rehydration. In this study, we aim to improve the stability of bFGF coacervates during freeze-drying to enable a topically applied growth factor delivery system for diabetic foot ulcer. Trehalose, mannitol, and Tween 80 at various concentrations were tested as cryoprotectant candidates. Trehalose improved the mechanical property of freeze-dried coacervates and physical properties after rehydration, resulting in stable size and PDI values. It also enh

SUBMITTER: Lee J 

PROVIDER: S-EPMC9781200 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature