Dataset Information

Genome-Based Analysis of Virulence Factors and Biofilm Formation in Novel P. aeruginosa Strains Isolated from Household Appliances.

ABSTRACT: In household washing machines, opportunistic pathogens such as Pseudomonas aeruginosa are present, which represent the household as a possible reservoir for clinical pathogens. Here, four novel P. aeruginosa strains, isolated from different sites of household appliances, were investigated regarding their biofilm formation. Only two isolates showed strong surface-adhered biofilm formation. In consequence of these phenotypic differences, we performed whole genome sequencing using Oxford Nanopore Technology together with Illumina MiSeq. Whole genome data were screened for the prevalence of 285 virulence- and biofilm-associated genes as well as for prophages. Linking biofilm phenotypes and parallelly appearing gene compositions, we assume a relevancy of the las quorum sensing system and the phage-encoded bacteriophage control infection gene bci, which was found on integrated phi297 DNA in all biofilm-forming isolates. Additionally, only the isolates revealing strong biofilm formation harbored the ϕCTX-like prophage Dobby, implicating a role of this prophage on biofilm formation. Investigations on clinically relevant pathogens within household appliances emphasize their adaptability to harsh environments, with high concentrations of detergents, providing greater insights into pathogenicity and underlying mechanisms. This in turn opens the possibility to map and characterize potentially relevant strains even before they appear as pathogens in society.

SUBMITTER: Kiel A

PROVIDER: S-EPMC9781345 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature

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Genome-Based Analysis of Virulence Factors and Biofilm Formation in Novel P. aeruginosa Strains Isolated from Household Appliances.

Kiel Annika A Creutz Ines I Rückert Christian C Kaltschmidt Bernhard Peter BP Hütten Andreas A Niehaus Karsten K Busche Tobias T Kaltschmidt Barbara B Kaltschmidt Christian C

Microorganisms 20221219 12

In household washing machines, opportunistic pathogens such as Pseudomonas aeruginosa are present, which represent the household as a possible reservoir for clinical pathogens. Here, four novel P. aeruginosa strains, isolated from different sites of household appliances, were investigated regarding their biofilm formation. Only two isolates showed strong surface-adhered biofilm formation. In consequence of these phenotypic differences, we performed whole genome sequencing using Oxf ...[more]

PMID: 36557761

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Project description:Bovine mastitis, a prevalent disease, is often attributed to staphylococci species. These microorganisms can express a diverse array of virulence genes and have the capability to form biofilms, establishing a robust defense against antimicrobials and host immune responses. In this study, we analyzed 191 Staphylococcus spp., of which 81 % were identified as Staphylococcus aureus, and 19 % as non-aureus staphylococci (NAS), including species such as S. borealis, S. chromogenes, S. haemolyticus, S. saprophyticus, S. capitis, S. ratti, and S. pasteuri. Our analysis involved determining antimicrobial susceptibility profiles, assessing biofilm-forming capacities, and identifying genes associated with virulence, biofilm formation, adhesion, and antimicrobial resistance. Notably, 17.2 % of the strains exhibited resistance to penicillin, with 97 % carrying the blaZ gene, while 9.4 % demonstrated resistance to erythromycin. All strains were sensitive to gentamicin and cefoxitin. Additionally, resistance was observed for clindamycin (8.4 %) and tetracycline (1.0 %). Concerning biofilm development, 2.6 % displayed no formation, 24.6 % were categorized as weak producers, 47.1 % as moderate, and 25.7 % as strong formers. Our investigation also unveiled the presence of virulence genes, such as superantigens like sea (4.7 %), seb (3.7 %), sec (8.4 %), sed (0.5 %), and tst (6.8 %); Panton-Valentine leukocidin (pvl) (59.7 %); haemolysins hla (88.5 %) and hlb (91.1 %); genes responsible for biofilm production icaA (87.9 %), icaD (78.5 %), and bap (4.2 %); and adhesion genes fnbpA (89.5 %), fnbpB (20.4 %), and clfA (89.0 %). Additionally, the strains were categorized into four groups based on their virulence attributes, revealing differences between S. aureus and NAS, with the latter showing a lower presence of the studied genes compared to S. aureus strains. This research sheds light on the resistance and virulence profiles of staphylococci strains associated with bovine mastitis, providing valuable insights for potential treatment approaches.

Dataset Information

Genome-Based Analysis of Virulence Factors and Biofilm Formation in Novel P. aeruginosa Strains Isolated from Household Appliances.

Publications

Genome-Based Analysis of Virulence Factors and Biofilm Formation in Novel <i>P. aeruginosa</i> Strains Isolated from Household Appliances.

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