Comparison of Bioluminescent Substrates in Natural Infection Models of Neglected Parasitic Diseases.

ABSTRACT: The application of in vivo bioluminescent imaging in infectious disease research has significantly increased over the past years. The detection of transgenic parasites expressing wildtype firefly luciferase is however hampered by a relatively low and heterogeneous tissue penetrating capacity of emitted light. Solutions are sought by using codon-optimized red-shifted luciferases that yield higher expression levels and produce relatively more red or near-infrared light, or by using modified bioluminescent substrates with enhanced cell permeability and improved luminogenic or pharmacokinetic properties. In this study, the in vitro and in vivo efficacy of two modified bioluminescent substrates, CycLuc1 and AkaLumine-HCl, were compared with that of D-luciferin as a gold standard. Comparisons we

SUBMITTER: Hendrickx S 

PROVIDER: S-EPMC9781651 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature