Fitness of mCherry Reporter Tick-Borne Encephalitis Virus in Tick Experimental Models.

ABSTRACT: The tick-borne encephalitis virus (TBEV) causes a most important viral life-threatening illness transmitted by ticks. The interactions between the virus and ticks are largely unexplored, indicating a lack of experimental tools and systematic studies. One such tool is recombinant reporter TBEV, offering antibody-free visualization to facilitate studies of transmission and interactions between a tick vector and a virus. In this paper, we utilized a recently developed recombinant TBEV expressing the reporter gene mCherry to study its fitness in various tick-derived in vitro cell cultures and live unfed nymphal Ixodes ricinus ticks. The reporter virus was successfully replicated in tick cell lines and live ticks as confirmed by the plaque assay and the mCherry-specific polymerase

SUBMITTER: Kevely A 

PROVIDER: S-EPMC9781894 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature