Combined Application of Orthogonal Sortases and Depsipeptide Substrates for Dual Protein Labeling.

ABSTRACT: Staphylococcus aureus sortase A is a transpeptidase that has been extensively exploited for site-specific modification of proteins and was originally used to attach a labeling reagent containing an LPXTG recognition sequence to a protein or peptide with an N-terminal glycine. Sortase mutants with other recognition sequences have also been reported, but in all cases, the reversibility of the transpeptidation reaction limits the efficiency of sortase-mediated labeling reactions. For the wildtype sortase, depsipeptide substrates, in which the scissile peptide bond is replaced with an ester, allow effectively irreversible sortase-mediated labeling as the alcohol byproduct is a poor competing nucleophile. In this paper, the use of depsipeptide substrates for evolved sortase variants is r

SUBMITTER: Morgan HE 

PROVIDER: S-EPMC9782347 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature