Confounding effects of liquorice, hydrocortisone, and blood contamination on salivary cortisol but not cortisone.

ABSTRACT:

Objective

To determine the effects of liquorice consumption, topical hydrocortisone, and blood contamination on salivary cortisol and cortisone concentrations.

Design and methods

Thirty healthy volunteers were randomized to a low, medium, or high dose of liquorice. Late-night saliva samples were collected using a Salivette® collection device at baseline, during 1 week of daily liquorice consumption, and during 4 weeks' washout. Saliva sampling was also performed before and after the application of topical hydrocortisone on the skin. Furthermore, in a subgroup (n = 16), saliva and venous blood were collected from each individual and mixed to achieve graded blood contamination in saliva. Salivary cortisol and cortisone were analyzed with liquid chromatography-tandem mass spect

SUBMITTER: Imamovic M 

PROVIDER: S-EPMC9782436 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature