A Green Blue LED-Driven Two-Liquid-Phase One-Pot Procedure for the Synthesis of Estrogen-Related Quinol Prodrugs
Ontology highlight
ABSTRACT:
SUBMITTER:
PROVIDER: S-EPMC9782465 | biostudies-literature | 2022 Dec
REPOSITORIES: biostudies-literature
Similar Datasets
Project description:The purpose of this study is to investigate whether LED light at different wavelength can improve wound healing in normal fibroblast. In this study, both diabetic and normal fibroblast cell lines were cultured and created some artificial wounds with pipette tips. After LED irradiation and microarray analysis, we found that GPCR Class A, a rhodopsin-like-structure gene, was significantly up regulated in all treated groups. Besides, for normal cell treated groups, the expression of other genes relevant to defense response to virus became strongly higher. However, in diabetic cells, genes relating to acute inflammatory response and mitotic cell cycle were highly expressed. Researchers also found that diabetic cells significantly respond to wound healing better than normal cells because inflammatory response, cell migration, cell proliferation, cell adhesion, and regulation of mitosis pathways can be instantly activated by red and green LED light according to gene expression profiles.
2016-02-18 | GSE78018 | GEO
Project description:The purpose of this study is to investigate whether LED light at different wavelength can improve wound healing in both diabetic fibroblast. In this study, both diabetic and normal fibroblast cell lines were cultured and created some artificial wounds with pipette tips. After LED irradiation and microarray analysis, we found that GPCR Class A, a rhodopsin-like-structure gene, was significantly up regulated in all treated groups. Besides, for normal cell treated groups, the expression of other genes relevant to defense response to virus became strongly higher. However, in diabetic cells, genes relating to acute inflammatory response and mitotic cell cycle were highly expressed. Researchers also found that diabetic cells significantly respond to wound healing better than normal cells because inflammatory response, cell migration, cell proliferation, cell adhesion, and regulation of mitosis pathways can be instantly activated by red and green LED light according to gene expression profiles.
2016-02-18 | GSE78017 | GEO
Project description:The purpose of this study is to investigate whether LED light at different wavelength can improve wound healing in normal fibroblast. In this study, both diabetic and normal fibroblast cell lines were cultured and created some artificial wounds with pipette tips. After LED irradiation and microarray analysis, we found that GPCR Class A, a rhodopsin-like-structure gene, was significantly up regulated in all treated groups. Besides, for normal cell treated groups, the expression of other genes relevant to defense response to virus became strongly higher. However, in diabetic cells, genes relating to acute inflammatory response and mitotic cell cycle were highly expressed. Researchers also found that diabetic cells significantly respond to wound healing better than normal cells because inflammatory response, cell migration, cell proliferation, cell adhesion, and regulation of mitosis pathways can be instantly activated by red and green LED light according to gene expression profiles. Normal cell lines were irradiated with different wavelength (red/green/blue) LED color and compare their gene expression with the control groups.
2016-02-18 | E-GEOD-78018 | biostudies-arrayexpress
Project description:The purpose of this study is to investigate whether LED light at different wavelength can improve wound healing in both diabetic fibroblast. In this study, both diabetic and normal fibroblast cell lines were cultured and created some artificial wounds with pipette tips. After LED irradiation and microarray analysis, we found that GPCR Class A, a rhodopsin-like-structure gene, was significantly up regulated in all treated groups. Besides, for normal cell treated groups, the expression of other genes relevant to defense response to virus became strongly higher. However, in diabetic cells, genes relating to acute inflammatory response and mitotic cell cycle were highly expressed. Researchers also found that diabetic cells significantly respond to wound healing better than normal cells because inflammatory response, cell migration, cell proliferation, cell adhesion, and regulation of mitosis pathways can be instantly activated by red and green LED light according to gene expression profiles. Diabetic fibroblast cells were irradiated with different wavelength (red/green/blue) LED color and compare their gene expression with the control groups.
2016-02-18 | E-GEOD-78017 | biostudies-arrayexpress
Project description:Substantial evidence now exists that intrinsic free-radical scavenging contributes to the receptor-independent neuroprotective effects of estrogens. This activity is inherently associated with the presence of a phenolic A-ring in the steroid. We report a previously unrecognized antioxidant cycle that maintains the "chemical shield" raised by estrogens against the most harmful reactive oxygen species, the hydroxyl radical (*OH) produced by the Fenton reaction. In this cycle, the capture of *OH was shown to produce a nonphenolic quinol with no affinity to the estrogen receptors. This quinol is then rapidly converted back to the parent estrogen via an enzyme-catalyzed reduction by using NAD(P)H as a coenzyme (reductant) and, unlike redox cycling of catechol estrogens, without the production of reactive oxygen species. Due to this process, protection of neuronal cells against oxidative stress is also possible by quinols that essentially act as prodrugs for the active hormone. We have shown that the quinol obtained from a 17beta-estradiol derivative was, indeed, able to attenuate glutamate-induced oxidative stress in cultured hippocampus-derived HT-22 cells. Estrone quinol was also equipotent with its parent estrogen in reducing lesion volume in ovariectomized rats after transient middle carotid artery occlusion followed by a 24-h reperfusion. These findings may establish the foundation for a rational design of neuroprotective antioxidants focusing on steroidal quinols as unique molecular leads.
| S-EPMC208828 | biostudies-literature
Project description:Re-designing existing nano-silver technologies to optimize efficacy and sustainability has a tangible impact on preventing infections and limiting the spread of pathogenic microorganisms. Advancements in manufacturing processes could lead to more cost-effective and scalable production methods, making nano-silver-based antimicrobial products more accessible in various applications, such as medical devices, textiles, and water purification systems. In this paper, we present a new, versatile, and eco-friendly one-pot process for preparing silver nanoparticles (AgNPs) at room temperature by using a quaternary ammonium salt of hydroxyethyl cellulose (HEC), a green ingredient, acting as a capping and reducing agent. The resulting nano-hybrid phase, AgHEC, consists of AgNPs embedded into a hydrogel matrix with a tunable viscosity depending on the conversion grade, from ions to nanoparticles, and on the pH. To investigate the synthesis kinetics, we monitored the reaction progress within the first 24 h by analyzing the obtained NPs in terms of particle size (dynamic light scattering (DLS), field emission scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM)), Z-potential (ELS), surface plasmon resonance (UV-VIS), crystallographic phase (XRD), viscosity, and reaction yield (inductively coupled plasma-optical emission spectrometry (ICP-OES)). To explore the design space associated with AgHEC synthesis, we prepared a set of sample variants by changing two independent key parameters that affect nucleation and growth steps, thereby impacting the physicochemical properties and the investigated antimicrobial activity. One of the identified design alternatives pointed out an improved antimicrobial activity in the suspension, which was confirmed after application as a coating on nonwoven cellulose fabrics. This enhancement was attributed to a lower particle size distribution and a positive synergistic effect with the HEC matrix.
| S-EPMC11349673 | biostudies-literature
Project description:Spanish fir (Abies pinsapo) is a relict tree that is found in southern Spain mountains. In natural conditions two phenotypes can be observed, trees with blue needles and trees with green needles. The aim of the present work is to elucidate at transcriptomic levels the possible causes of the differences between these phenotypes.
2021-11-20 | GSE189122 | GEO
Project description:In the directed evolution experiments of EGFP or StayGold, the objective was to blue-shift the excitation light spectrum of GFP towards shorter wavelengths, resulting in an enhancement of fluorescence intensity upon 405 nm laser excitation. This facilitates the advancement of novel fluorescent proteins. These experiments represent a category of experiments that utilize REPLACE to achieve discontinuous directed evolution through FACS sorting (screening).
2024-09-30 | GSE271876 | GEO
Project description:In this work, silver nanoparticles (AgNPs) were synthesized from cashew nutshell liquid (CNSL) by varying the concentration of silver ions and the pH of the CNSL extract. The synthesized AgNPs were further characterized to study their surface, structural, and morphological properties and tested for the removal of methylene blue (MB) dye. The results of this study showed that depending on the conditions, particles of various sizes, ranging from 1 to 60 nm, and different degrees of stabilization and agglomeration were produced. The concentration of silver ions equal to 3 mM and the pH of the extract of ~4.5 (AgNP3) resulted in the most efficient synthesis, where particles appeared to be highly stabilized and homogeneously distributed on the surface, exhibiting a small average particle size and a narrow particle size distribution (6.7 ± 6.5 nm). Such particles further showed the highest percent removal of MB, where up to 80% removal was recorded within the first 20 min. Higher concentrations of silver ions and higher pH of the extract resulted in substantial particle agglomeration and particles being over-capped by the CNSL biomolecules, respectively, which further negatively affected the ability of particles to remove MB. Finally, the fact that visible light showed no significant effect on the removal of MB, with the average removal rates found to be about the same as in the dark, suggests the strong catalytic nature of AgNPs, which facilitates the electron transfer reactions leading to MB reduction.
| S-EPMC11357457 | biostudies-literature
Project description:Liquid fertilizers, particularly when integrated with precision irrigation systems, offer a more efficient and sustainable alternative to traditional solid nitrogen fertilizers. The industrial production of ammonium nitrate (NH4NO3) is environmentally detrimental due to its reliance on fossil fuels. This study introduces an innovative air-to-NOx-to-NH4NO3 pathway for synthesizing liquid nitrogen fertilizer. The process employs an underwater multi-bubble plasma reactor powered by nanosecond pulse to generate aqueous NOx, which is then partially reduced to NH4NO3 through electrocatalysis. Results show that the highest NOx production rate, 786.5 mol h-1, is achieved when the N2/O2 ratio closely resemble that of air, and short pulse rise/fall times significantly increase NOx yield. Further plasma diagnostic and global plasma chemistry modeling indicate that short rise/fall times facilitate simultaneous dielectric barrier discharge and spark discharge, synergistically enhancing nitrogen fixation efficiency. The partially electro-reduced liquid NH4NO3 fertilizer significantly improves plant growth, with stem length and leaf length increasing by 91.26% and 54.72%, respectively. Cost estimation reveals that 44.22% of the production cost is attributed to electricity consumption, underscoring the potential for optimization with renewable energy integration. Overall, the study provides new insight for the sustainable production and in-place utilization of liquid nitrogen fertilizers which may advance sustainable agriculture.
| S-EPMC11848547 | biostudies-literature