Cefsulodin and Vancomycin: A Supplement for Chromogenic Coliform Agar for Detection of <i>Escherichia coli</i> and Coliform Bacteria from Different Water Sources.

ABSTRACT: Background microorganism growth on Chromogenic Coliform Agar (CCA) can be challenging. For this reason, an alternative method with a Cefsulodin/Vancomycin (CV)-supplemented CCA should be evaluated regarding its application in water from different sources. CCA supplemented with CV was validated according to ÖNORM EN ISO 16140-4:2021 using water from natural sources in Styria, Austria. Results show that the alternative method using the supplemented CCA has similar values in relation to sensitivity (82.2%), specificity (98.6%) and higher selectivity (59%) compared to the reference method. Repeatability and reproducibility were acceptable for the alternative method and showed similar results with the reference method. The alternative method shows a very low false positive rate and a low false

SUBMITTER: Schalli M 

PROVIDER: S-EPMC9783415 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature