Chlamydia abortus Isolation and Identification in Aborted Ovine Fetus in Mari El Republic of Russia.

ABSTRACT: Reproductive disorders, presumably caused by Chlamydia abortus, are common among the ovine population of the Mari El Republic, Russia. C. abortus infection was determined by serologic testing or isolation and detection of the organism by PCR and direct immunofluorescence in tissue samples. Rams, ewes, and lambs (10 individuals each) were randomly chosen for serological testing by the complement fixation test and 7 of 30 (23%) animals tested were positive. Tissue samples were collected from ewes and aborted fetuses for isolation by inoculating chicken embryo yolk sacs (n = 41). The same samples were analyzed by PCR using commercial and in-house PCR kits and by direct immunofluorescence. C. abortus was detected in 58.5% of samples using PCR and in 60.9% of the sam

SUBMITTER: Matveeva I 

PROVIDER: S-EPMC9784139 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature