Effect of Superovulation Treatment on Oocyte's DNA Methylation.

ABSTRACT: Controlled ovarian stimulation is a necessary step in some assisted reproductive procedures allowing a higher collection of female gametes. However, consequences of this stimulation for the gamete or the offspring have been shown in several mammals. Most studies used comparisons between oocytes from different donors, which may contribute to different responses. In this work, we use the bovine model in which each animal serves as its own control. DNA methylation profiles were obtained by single-cell whole-genome bisulfite sequencing of oocytes from pre-ovulatory unstimulated follicles compared to oocytes from stimulated follicles. Results show that the global percentage of methylation was similar between groups, but the percentage of methylation was lower for non-stimulated oocytes in the i

SUBMITTER: Lopes JS 

PROVIDER: S-EPMC9785075 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature