Proteomics Evaluation of Semen of Clinically Healthy Beagle-Breed Dogs.

ABSTRACT: The objectives of the present work were to evaluate the semen of dogs by means of proteomics methods and to compare with proteomics results of the blood of the animals, in order to increase available knowledge on the topic and present relevant reference values for semen samples. Semen samples were collected from five Beagle-breed dogs. Reproductive assessment of the animals by means of clinical, ultrasonographic and seminological examinations confirmed their reproductive health. The sperm-rich fraction and the prostatic fraction of semen were processed for proteomics evaluation. LC-MS/MS analysis was performed by means of a LTQ Orbitrap Elite system. The technology combines high separation capacity and strong qualitative ability of proteins in biological samples that require deep proteome

SUBMITTER: Gouletsou PG 

PROVIDER: S-EPMC9785154 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature