Improved Expression of SARS-CoV-2 Spike RBD Using the Insect Cell-Baculovirus System.

ABSTRACT: Insect cell-baculovirus expression vector system is one of the most established platforms to produce biological products, and it plays a fundamental role in the context of COVID-19 emergency, providing recombinant proteins for treatment, diagnosis, and prevention. SARS-CoV-2 infection is mediated by the interaction of the spike glycoprotein trimer via its receptor-binding domain (RBD) with the host's cellular receptor. As RBD is required for many applications, in the context of pandemic it is important to meet the challenge of producing a high amount of recombinant RBD (rRBD). For this reason, in the present study, we developed a process based on Sf9 insect cells to improve rRBD yield. rRBD was recovered from the supernatant of infected cells and easily purified by metal ion affinity chrom

SUBMITTER: Poodts J 

PROVIDER: S-EPMC9785345 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature