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Versatile Confocal Raman Imaging Microscope Built from Off-the-Shelf Opto-Mechanical Components.


ABSTRACT: Confocal Raman microscopic (CRM) imaging has evolved to become a key tool for spatially resolved, compositional analysis and imaging, down to the μm-scale, and nowadays one may choose between numerous commercial instruments. That notwithstanding, situations may arise which exclude the use of a commercial instrument, e.g., if the analysis involves toxic or radioactive samples/environments; one may not wish to render an expensive instrument unusable for other uses, due to contamination. Therefore, custom-designed CRM instrumentation-being adaptable to hazardous conditions and providing operational flexibility-may be beneficial. Here, we describe a CRM setup, which is constructed nearly in its entirety from off-the-shelf optomechanical and optical components. The original aim was to develop a

SUBMITTER: Diaz Barrero D 

PROVIDER: S-EPMC9786121 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature