An Efficient and Economical N-Glycome Sample Preparation Using Acetone Precipitation.

ABSTRACT: Due to the critical role of the glycome in organisms and its close connections with various diseases, much time and effort have been dedicated to glycomics-related studies in the past decade. To achieve accurate and reliable identification and quantification of glycans extracted from biological samples, several analysis methods have been well-developed. One commonly used methodology for the sample preparation of N-glycomics usually involves enzymatic cleavage by PNGase F, followed by sample purification using C18 cartridges to remove proteins. PNGase F and C18 cartridges are very efficient both for cleaving N-glycans and for protein removal. However, this method is most suitable for a limited quantity of samples. In this study, we developed a sample preparation method focusin

SUBMITTER: Wang J 

PROVIDER: S-EPMC9786591 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature