Nerve Targeting via Myelin Protein Zero and the Impact of Dimerization on Binding Affinity.

ABSTRACT:

Background

Surgically induced nerve damage is a common but debilitating side effect. By developing tracers that specifically target the most abundant protein in peripheral myelin, namely myelin protein zero (P0), we intend to support fluorescence-guided nerve-sparing surgery. To that end, we aimed to develop a dimeric tracer that shows a superior affinity for P0.

Methods

Following truncation of homotypic P0 protein-based peptide sequences and fluorescence labeling, the lead compound Cy5-P0_101-125 was selected. Using a bifunctional fluorescent dye, the dimeric Cy5-(P0_101-125)₂ was created. Assessment of the performance of the mono- and bi-labeled compounds was based on (photo)physical evaluation. This was followed by in vitro assessment in P0

SUBMITTER: Berehova N 

PROVIDER: S-EPMC9786614 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature