Metabolic Engineering of Nicotiana benthamiana to Produce Cannabinoid Precursors and Their Analogues.

ABSTRACT: In recent years, the perspective towards the use of cannabis has slowly shifted from being an illicit drug to a medicinal plant. The pathway and enzymes involved in the production of cannabinoids are known; however, studies evaluating the production of cannabinoids in heterologous plants and cell cultures are still limited. In this study, we assessed the potential use of N. benthamiana (Nicotiana benthamiana) plants as a heterologous host for producing natural and novel cannabinoids. Transgenic N. benthamiana plants expressing genes encoding cannabis acyl-activating enzyme and olivetol synthase were generated, which were then used for transiently expressing other downstream pathway genes. Production of olivetolic acid and divarinic acid, the universal precursors for ma

SUBMITTER: Reddy VA 

PROVIDER: S-EPMC9786632 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature